Journal: eLife

Article Title: The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack

doi: 10.7554/eLife.41301

Figure Lengend Snippet:

Article Snippet: Cell line ( Rattus norvegicus ) , Normal rat kidney (NRK) fibroblast cell , ATCC , ATCC: CRL-1570; RRID: CVCL_2144 , .

Techniques: Recombinant, Plasmid Preparation, Antibody Labeling, Software

Journal: PeerJ

Article Title: Melatonin increases growth properties in human dermal papilla spheroids by activating AKT/GSK3β/β-Catenin signaling pathway

doi: 10.7717/peerj.13461

Figure Lengend Snippet: Human epidermal keratinocyte (HEK), human dermal fibroblast (HDF), and human dermal papilla (HDP) cells were incubated for 48 h. (A) The mRNA levels of MTNR1A and MTNR1B were assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and GAPDH served as an endogenous control. (B) Protein levels of MTNR1A and MTNR1B were assessed by western blotting, and β-actin served as a loading control. (C) HDP cells were incubated for 48 h in 2D and 3D culture systems, and mRNA levels of MTNR1A and MTNR1B were assessed by qRT-PCR. GAPDH served as an endogenous control. Data are presented as the mean ± standard deviation (SD) of three independent experiments, and normally distributed data were evaluated using two-way analysis of variance (ANOVA), followed by Tukey’s post hoc test; ** p < 0.01, **** p < 0.0001 versus HDP control cells ( p = 0.0019, p = 0.0014, p < 0.0001, and p < 0.0001, respectively) (A), and ** p < 0.01 versus 2D cultured HDP control cells ( p = 0.0052) (C).

Article Snippet: Human epidermal keratinocyte (HEK) cells (CELLnTEC, Bern, Switzerland) were maintained in CnT-Prime Epithelial Culture Medium (CELLnTEC) in 5% CO 2 at 37 °C.

Techniques: Incubation, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Control, Western Blot, Standard Deviation, Cell Culture

Journal: PLoS ONE

Article Title: Analysis of Altered MicroRNA Expression Profiles in Proximal Renal Tubular Cells in Response to Calcium Oxalate Monohydrate Crystal Adhesion: Implications for Kidney Stone Disease

doi: 10.1371/journal.pone.0101306

Figure Lengend Snippet: Analysis of biological processes of the predicted miRNAs targets by GOstat.

Article Snippet: Human Kidney Epithelial Cells, HK-2, were procured from American Type Culture Collection (ATCC) and maintained in a DMEM medium supplemented with 10% Fetal Bovine Serum and antibiotics.

Techniques: Activity Assay, Binding Assay